Ajinomoto Co., Ltd.

Other

Rustem Saidovich Shakulov

Elena Vitalievna Klyachko

Vitaly Arkadievich Livshits

Evgeny Moiseevich Khurges

Vera Georgievna Doroshenko

Vladimir Georgievich Debabov

Tatyana Alexandrovna Bachina

Yuri Ivanovich Kozlov

Larisa Gotlibovna Airikh

Aaveilova Oksaua Fedorovva

Zakataeva Natalya Pavlovva

Mikhail Markovich Gusyatiner

Sergei Vladimirovich Mashko

Nelli Isaakovna Zhdanova

Nikolai Kazimirovich Yankovsky

Jury I Kozlov

Raisa Alexandrovna Arsatiants

Vladimir G Debabov

Ljudmila F Kozyreva

Mikhail N Rozinov

Boris A Rebentish

Vera N Moshentseva

A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3'-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.

Method for producing lower alkyl ester

A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of thefamily, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3'-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.

L-amino acid producing bacterium

The present invention relates to a mutant bacterial PRPP synthetase which is resistant to feedback by purine nucleotides, and a method for producing L-histidine using the bacterium of the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by use of the PRPP synthetase which is resistant to feedback by purine nucleotides, coded by the mutant prsA gene.

Mutant phosphoribosylpyrophosphate synthetase and method for producing L-histidine

A method is provided for producing L-histidine using bacterium of the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the AICAR transformylase-IMP cyclohydrolase encoded by the purH gene.

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A method for constructing microorganism strains which produce amino acids comprising: combining in one bacterial genome (a) a mutation affecting the aminoacyl-tRNA synthetase corresponding to the selected amino acid, conferring cells auxotrophy which cannot be fully suppressed by addition of usual concentration this amino acid into the culture medium and (b) a mutation which destroys the negative regulation of selected amino acid biosynthesis to yield a strain capable of increased production of the selected amino acid. Strains producing isoleucine and valine. Methods to produce isoleucine and valine by culturing those strains.

Strains of Escherichia coli which produce isoleucine or valine and a

The present invention provides strains of Escherichia coli which have an isoleucine-tRNA synthetase with an increased Km and no negative feedback inhibition of isoleucine production. These strains are effective for the production of isoleucine in high amounts.

Production of isoleucine by escherichia coli having isoleucine

A method for constructing strains which produce aminoacids comprising combining of a DNA chromosome fragment of a donor microorganism containing genes controlling the synthesis of a selected aminoacid and having a mutation destroying the negative regulation of the synthesis of this aminoacid with a vector DNA molecule to form a hybrid DNA molecule. Use is made of a vector DNA molecule capable of providing amplification of the hybrid DNA molecule. The resulting hybrid DNA molecule is used for transforming cells of the recipient strain having the mutation blocking the synthesis of the selected aminoacid in this strain and the mutation partly blocking the related step of metabolism of this aminoacid to yield the strain capable of increased productivity of the selected aminoacid.

Method for preparing strains which produce aminoacids

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Moscow, Russia

Rustem Saidovich Shakulov