Watchmaker Genomics, Inc.

Kapa Biosystems, Inc.

Ross Wadsworth

Bjarne Faurholm

Paul McEwan

Martin Ranik

Brian Kudlow

Eric Van Der Walt

Jennifer Hsieh

Julie Walker

Brian A Kudlow

Marliz Strydom

REVERSE TRANSCRIPTASE VARIANTS

The present invention provides MMLV reverse transcriptase enzymes with increased thermal stability as compared with wild type MMLV and AMV reverse transcriptases. The improved thermal stability allows for reverse transcription of RNA to cDNA at temperatures above 37&#xb0; C., thereby reducing error rates introduced during cDNA synthesis. As a result, the reverse transcriptases of the invention allow for increased accuracy in the determination of transcriptomes of living organisms.

Reverse transcriptase variants

The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5' to 3&#x2032;, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.

System and method for nucleic acid library preparation via template switching mechanism

Growing community of inventors

Cape Town, South Africa

Ross Wadsworth