Other

The University of Texas System

Valentis, Inc.

Am Biotechnologies, Inc.

Ross Durland

Samuel Nochumson

Rexford Hayes

Audrey Yu-Speight

John Welp

David G Gorenstein

Johnnie Engelhardt

Miguel-Angel Elizondo-Riojas

Kuowei Wu

Weiguo He

Kuoewi Wu

David E Volk

Provided herein are methods for a novel bead-based next-generation 'X-aptamer' selection scheme that extends aptamer technology to include X-modified bases, thus resulting in X-aptamers, at any position along the sequence because the aptamers are chemically synthesized via a split-pool scheme on individual beads. Also provides are application to a wide range of commonly used DNA modifications, including, but not limited to, monothioate and dithioate backbone substitutions. This new class of aptamer allows chemical modifications introduced to any of the bases in the aptamer sequence as well as the phosphate backbones and can be extended to other carbohydrate-based systems.

Methods of X-aptamer generation and compositions thereof

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinantcells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.

Process and equipment for plasmid purification

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinantcell. Effective, contgrollable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and nuetralization.

Growing community of inventors

The Woodlands, TX, United States of America

Ross Durland