Syngenta Participations Ag

Rogerio T N Prata

Michael G Willits

Gerson Graser

Catherine M Kramer

Vincenzo Deluca

Shib Sankar Basu

Myoung Kim

Xuejun Sean Zhong

Tricia Charlene Costello

Hangsik Moon

Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.

Method of measuring alpha amylase

The present invention includes non-transgenic domesticated tomato plants that express flavonols in the flesh and peel of the tomato fruit, and includes the seeds and fruits of such plants. The method of the invention includes assaying tomato plants for expression of one or more of the flavonol biosynthesis genes in the flesh and/or expression of CHI in the peel. The method of the invention includes selecting wild tomato species that express CHI in the fruit peel, and/or one or more genes of the flavonol biosynthesis pathway in the flesh, and introgressing the genetic factors responsible for this expression from the wild tomato species into a domesticated tomato plant using traditional breeding techniques.

Flavonol expressing domesticated tomato and method of production

Growing community of inventors

Chapel Hill, NC, United States of America

Rogerio T N Prata