Mayo Foundation for Medical Education and Research

Bundersrepublik Deutschland, Letzvertreten Durch Den Präsidenten Des Paul-ehrlich-instituts

Bundesrepublik Deutschland, Letztvertreten Durch Den Prasidenten Des Paul-ehrlich-instituts

Roberto Cattaneo

Stephen James Russell

Francois-Loic Cosset

Adele Kay Fielding

Frances Joanne Morling

Klaus Cichutek

Christian Buchholz

Vincent H J Leonard

Sompong Vongpunsawad

Sabrina Funke

Takafumi Nakamura

Sabrina Kneissl

The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation ofF and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.

Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high throughput screening

The document provides nucleic acids, polypeptides, and viruses containing nucleic acids and/or polypeptides. The document also provides methods for using viruses to treat cancer patients. Specifically, the document provides nucleic acid molecules encoding viral hemagglutinin (H) polypeptides, viral H polypeptides, and viruses containing nucleic acids and/or H polypeptides. Such viruses are useful for vaccinations and for treating cancer patients as the viruses are not shed.

Viruses lacking epithelial cell receptor entry

Disclosed are compositions comprising a recombinant nucleic acid vector including a nucleotide sequence encoding a syncytium-inducing polypeptide expressible on a eukaryotic cell surface, and a host cell containing the recombinant vector and expressing the syncytium inducing polypeptide on its cell surface, the vectors and resultant host cells expressing the syncytium inducing polypeptide being useful for selective elimination of unwanted cells.

Compositions and methods for elimination of unwanted cells

Control apparatus forcefully stops inverter and inverter when DC/DC converter is anomalously stopped. Additionally, when one of inverters is anomalously stopped while DC/DC converter is normal, control apparatus forcefully stops the other inverter. Then, when a recovery condition is satisfied after the other inverter is forcefully stopped, control apparatus recovers the other inverter.

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Rochester, MN, United States of America

Roberto Cattaneo