Cpc International, Inc.

Robert E Heady

Jeffrey R Frankiewicz

Leo R Idaszak

Frederick C Armbruster

Robert P Cory

William A Jacaway, Jr

Richard A Terranova

Jorge H Alonso

An improved process for the continuous production of butanol by fermentation of carbohydrates with C. acetobutylicum is disclosed. This process combines continuous inoculum production at a high dilution rate and cycling the fermentation broth through a material which adsorbs butanol whereby a vigorous cell population is maintained in the fermentation reactor for extended periods of time.

Production of butanol by a continuous fermentation process

A novel method for making n-butyl butyrate is disclosed. This method comprises passing a fermentation medium containing solvent-producing cells of C. acetobutylicum through a bed of activated carbon followed by desorbing the n-butyl butyrate from the carbon.

Process for making n-butyl butyrate

This invention relates generally to (1) processes for the production and isolation of a novel fructosyl transferase enzyme from the fermentation broth of Pullularia pullulans, (2) enzymatic transfructosylation of sucrose to produce a novel fructose-polymer containing substrate, and (3) production of fructose syrups containing greater than 55% fructose from said novel substrate.

Preparation of high fructose syrups from sucrose

This invention relates to a 1-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for production of high fructose syrups.

Process for the production of high fructose syrups and ethanol

This invention relates to a 2-step process for the preparation of fructose polymers and ethyl alcohol from sucrose. The fructose polymers are especially useful for the production of high fructose syrups.

This invention relates to a process especially useful for the preparation of novel fructose polymers and high fructose syrups from sucrose.

Process for the production of fructose polymers and high fructose syrups

Process for the production of xylose (dextrose) isomerase by means of a mutant strain of Streptomyces that proliferates in a culture medium that may be free of xylose. The xylose (dextrose) isomerase enzyme is therefore constitutive rather than induced.

Production of xylose (dextrose) isomerase enzyme preparations

Process of enzymatically converting glucose to fructose wherein a glucose-containing solution is passed, under specific conditions, through a bed composed of an immobilized glucose isomerase in a fine, particulate form and non-porous beads or granular inert material such as polystyrene beads. The porous beads and granular inert material comprise between 40% and about 75% of the bed by volume. The bead's presence exerts a buoyant effect which prevents bed packing and promotes desirable flow patterns with no observable channelling. This helps to minimize the formation of undesired or undesirable carbohydrates such as psicose.

Inert, non-porous granules for flow control in a plug flow reactor

Levulose-bearing syrups are produced by passing a dextrose solution through a column or bed of an immobilized dextrose isomerase enzyme preparation. The useful life of the enzyme preparation is extended by conducting the isomerization initially at a low operating temperature, then increasing the temperature of operation after an initial decrease in enzyme activity has been observed. Also disclosed is an improved immobilized xylose isomerase enzyme preparation which is prepared by contacting a solution of xylose isomerase with particulate basic magnesium carbonate. The novel immobilized enzyme preparation is useful in enzymatically isomerizing dextrose solutions to levulose-bearing syrups.

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Park Forest, IL, United States of America

Robert E Heady