Pharmacopeia Inc.

Robert A Horlick

Maria Webb

Alan K Robbins

Robert Swanson

Barbara Strohl

Jiuqiao Zhao

Daniel Chelsky

Bassam B Damaj

The genes for bradykinin B receptor from five mammalian species, vervet monkey, rhesus macaque, tree shrew, dog and pig have been cloned and characterized. In addition to the delineation of the nucleotide and amino acid sequences, methods for identifying modulators of bradykinin B receptor activity using these molecules is also described. Additionally, a method for identifying an animal model useful in the screening of potential therapeutic agents is provided.

Cloning and characterization of genes encoding bradykinin B1 receptor homologues from five mammalian species

A method for obtaining a eukaryotic cell transfected with an episome involves transfecting the cell with the episome under conditions wherein cells survive that are successfully transfected with the episome. The resulting cells express both a first protein whose expression causes cell death and a second protein whose expression prevents cell death resulting from expression of the first protein.

Method for maintenance and selection of episomes

A method is described for producing recombinant eukaryotic cell lines expressing multiple proteins of interest. Eukaryotic host cells are transfected with (a) a first episome which contains an EBV origin of replication and a first gene encoding a protein of interest; and (b) a second episome containing an EBV origin of replication and a second gene encoding a protein of interest. Transfected cells are obtained expressing an EBNA 1 protein. The cells are grown under conditions wherein the episomes express the first and second genes.

Eukaryotic cells stably expressing genes from multiple transfected

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Plainsboro, NJ, United States of America

Robert A Horlick