St. Jude Children's Research Hospital

Richard Jay Smeyne

Martine F Roussel

Frederique Zindy

Justine Cunningham

Amar K Pani

Neil Segil

Ping Chen

This invention relates is a label-free, enzyme-free, aptamer-free method for simultaneously measuring adenosine, and its intracellular metabolites, e.g., AMP, ADP and ATP, using high pressure liquid chromatography coupled to electrochemical detector (HPLC-ECD).

Method for detection of adenosine and metabolites thereof

Non-human animals that have been manipulated so as not to express a functional p19 and p27 proteins are described. One particular non-human animal exemplified is a p19 -double null, p27 -double null mouse. Such knockout mice exhibit bradykinesia, proprioceptive abnormalities, seizure-like activity, and generally die 14-24 days after birth. In addition, mammalian cells having the p19 -double null, p27 -double null genotype are characterized. Methods of making and using the non-human knockout animals and cells are disclosed.

Cells that lack p19ink4d and p27kip1 activity and methods of use thereof

Non-human animals that have been manipulated so as not to express a functional p19,and p27,proteins are described. One particulat non-human animal exemplified is a p19,-double null, p27,-double null mouse. Such knockout mice exhibit bradykinesia, proprioceptive abnormalties, seizure-like activity, and generally die 14-24 days after birth. In addition, mammalian cells having the p19,-double null, p27,-double null genotype are characterized. Methods of making and using the non-human knockout animals and cells are disclosed.

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Collierville, TN, United States of America

Richard Jay Smeyne