Sigma-aldrich Co. LLC

Richard J Mehigh

Handong Li

John G Dapron

William Karl Kappel

Venkatappa Viswanatha

Jeff Porter

Edward B Watson, Iii

Ian R Brockie

Vankatappa Viswanatha

Elizabeth Jenkins

Eliezer Kopf

Ronald A Hernan

Efrat Reem

The present invention provides lysis reagents, containers, methods and kits relating to the extraction or the extraction and isolation of a cellular component from a host cell. More specifically, the invention provides combinations of zwitterionic compounds that may be employed to aide in the extraction or the extraction and isolation of a cellular component from a host cell.

Method for extracting a target product from a host cell employing zwitterionic detergent combinations

This invention describes a process for separating a fusion protein or polypeptide in the form of its precursor from a mixture containing said fusion protein and impurities, which comprises contacting said fusion protein with a resin containing immobilized metal ions, said fusion protein covalently operably linked directly or indirectly to an immobilized metal ion-affinity peptide, binding said fusion protein to said resin, and selectively eluting said fusion protein from said resin.

Affinity peptides and method for purification of recombinant proteins

Processes for the detection and purification of peptides and proteins comprising a metal ion-affinity peptide having a high affinity for coordinated metals and also being highly antigenic are described. Antibodies for use in the processes are also described.

Processes for purification of recombinant proteins

A metal chelating composition having the formula: wherein

Metal chelating compositions for protein purification, detection or binding

Compositions and methods employing zwitterionic detergent combinations

A metal chelating composition having the formula:wherein

Metal chelating compositions

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Oakville, MO, United States of America

Richard J Mehigh