Rhone-poulenc Agrochimie

Richard De Rose

Georges L Freyssinet

Michel Lebrun

Claude Gigot

Dominique Job

Claudette Job

Roland Douce

Juan Rigau

Javier Uribe

Miguel A Torres

Pedro Puigdomenech

Lluis Montoliu

Rossitza Atanassova

Montserrat Capellades

Manuel Duval

Isolated DNA sequence which can serve as terminator region in a chimeric gene capable of being used for the transformation of plants

A biotinylated protein is disclosed which is obtained from the seeds of leguminous plants and which is expressed exclusively in the seeds and in no other tissue. The protein comprises at least one subunit of 50-85 kDa. Levels of the protein decrease rapidly as germination of the seed progresses. The protein does not exhibit the activity of either acetyl-CoA carboxylase or 3-methyl crotonyl-CoA carboxylase. In the pea, Pisum sativum, the protein is designated SBP65 and comprises 6-8 identical subunits, each having a molecular weight of about 65 kDa. The protein may be a useful marker for determining the germination stage of seeds.

Seed specific biotinylated protein, SBP65, from leguminous plants

Regulatory regions from maize .alpha.-tubulin genes are disclosed. In particular, 5' regulatory regions comprising an upstream regulatory ensemble (URE) from maize .alpha.-tubulin 1 and 3 genes is useful in tissue specific expression of heterologous genes in transformed plants. The maize .alpha.-tubulin URE comprises regulatory elements which when operably linked to a promoter and heterologous gene, confer regulated expression in roots, pollen and meristematic tissues. Expression constructs which confer tissue specific expression are also provided.

Promoter elements of chimeric genes of .alpha.-tubulin

Growing community of inventors

Lyons, France

Richard De Rose