Neste Oil Oyj

Neste Oyj

Neste Corporation

Suomen Ymparistokeskus Syke

Fortum Oyj

Reijo Tanner

Perttu Koskinen

Annika Malm

Simo Laakso

Mervi Hujanen

Heidi Vainio

Miia Laamanen

Jukka Seppälä

Pasi Ylöstalo

Pauliina Uronen

Kristian Spilling

Timo Tamminen

Raisa Vermasvuori

The present invention relates to improved methods for producing biosynthetic products in a cascade of bioreactors. In particular the present invention relates to methods and a cascade of bioreactor systems comprising at least two bioreactors and at least one concentration unit.

Methods for continuous production of products from microorganisms

The present description is related to the field of cultivating algae. It introduces a method of cultivating algae by depleting the culture of an inorganic nutrient and exposing the alga to high intensity light to obtain algal cell mass having enriched lipid content and reduced chlorophyll content.

Method of cultivating algae

Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.

Integrated process for producing biofuels

The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.

Integrated process system for single cell oil production and a pulp and/or paper industry process

A method for recovering lipids from microbial biomass, the method including providing wet microbial biomass which contains lipids to extraction without disrupting the biomass cell walls, and subsequently, extracting the wet microbial biomass with a liquid extractant at elevated temperature of at least 170&#xb0; C. and elevated pressure. The combination of the temperature and pressure is such that the lipids in the cells are contacted with the extractant. Subsequently the extracted lipids are recovered from or with the extractant.

Method for lipid extraction from biomass

The present invention relates to a method for purification of lipid material originating from biological material. In the method the lipid material comprising acylglycerols and phosphorus impurities and at least one added nonpolar solvent and at least one added polar solvent is provided into a reaction zone whereby at least a two phase system comprising a nonpolar phase and a polar phase is formed. The phase system is heated in the closed reaction zone under mixing at a temperature from 150&#xb0; C. to 300&#xb0; C. and at a pressure wherein said solvents are in subcritical state, preferably of below 100 bar, dependent on the vapor pressure of the selected solvents, until the phosphorus impurity is removed from the polar phase. Subsequently, the nonpolar phase including the purified oil comprising acylglycerols is separated and recovered from said phase system.

Method for purifying lipid material

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Hikia, Finland

Reijo Tanner