Orna Therapeutics, Inc.

Massachusetts Institute of Technology

Raffaella Squilloni

Daniel Griffith Anderson

Robert Alexander Wesselhoeft

Allen T Horhota

Brian Goodman

Shinichiro Fuse

Circular RNA and transfer vehicles, along with related compositions and methods are described herein. In some embodiments, the inventive circular RNA comprises group I intron fragments, spacers, an IRES, duplex forming regions, and an expression sequence. In some embodiments, the expression sequence encodes a chimeric antigen receptor (CAR). In some embodiments, circular RNA of the invention has improved expression, functional stability, immunogenicity, ease of manufacturing, and/or half-life when compared to linear RNA. In some embodiments, inventive methods and constructs result in improved circularization efficiency, splicing efficiency, and/or purity when compared to existing RNA circularization approaches.

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Cambridge, MA, United States of America

Raffaella Squilloni