Cargill Inc.

Rohm Enzyme Finland Oy

Ab Enzymes Oy

Alliance for Sustainable Energy, LLC

Other

Natureworks LLC

Ab Enzymes Gmbh

Alko Ltd.

Alko-yhtiot Oy

R&ouml;hm Enzyme Finland Oy

Pirkko Suominen

Marja T Paloheimo

Raija Lantto

Aristos Aristidou

Kari Koivuranta

Vineet Rajgarhia

Laura Ruohonen

Tarja Lahtinen

Marja Ilmen

Arja Mantyla

Jari Vehmaanper&auml;

Christopher K Miller

Stacey Olson

Arja M&auml;ntyl&auml;

Merja Penttilä

John Londesborough

Richard B Fagerstrom

Heli Haakana

Vesa Joutsjoki

Arja Miettinen-Oinonen

Minna Elovainio

Richard Fagerstr&ouml;m

Eric E Jarvis

Min Zhang

Mary Ann Franden

Eric P Knoshaug

Arjun Singh

Holly J Jessen

Merja Elisa Penttila

Hans H Liao

Catherine Asleson Dundon

Ana Negrete-Raymond

Benjamin Matthew Hause

Helena Nevalainen

Kevin Roberg-Perez

Jian Yi

Joshua Lundorff

Merja Pentilla

Satu Hakola

Brian Jeffrey Rush

Jari Vehmaanperä

Terhi Puranen

Jarno Kallio

Thomas W McMullin

Chris Miller

Pim Van Hoek

Jonathan Knowles

Marja Ilmén

Richard Fagerström

Aria M&auml;ntyl&auml;

Richard B Fagerstr&ouml;m

Raija Lanto

Sanna Leskinen

Ben Hause

Ritva Saarelainen

Arja Mäntylä

Matthew Miller

Thomas William McMULLIN

Merja Penttilae

Merja Pentillä

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the generaor. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

The present application discloses the identification of the novelxylose transporter genes KHT105 and RAG4, as well as the identification of a novel set ofpentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

Compositions and methods for increased ethanol production from biomass

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.

L-arabinose fermenting yeast

Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced L- or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.

Lactic acid-producing yeast cells having nonfunctional L- or D-lactate:ferricytochrome C oxidoreductase cells

Cells of the speciesand closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

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Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species and fermentation processes using genetically modified yeast

An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains. 

The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. inand. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g. in pulp and paper industries.

Production and secretion of proteins of bacterial origin in filamentous fungi

Recombinant yeast are provided having, in one aspect, multiple exogenous LDH genes integrated into the genome, while leaving native PDC genes intact. In a second aspect, recombinant yeast are provided having an exogenous LDH gene integrated into its genome at the locus of a native PDC gene, with deletion of the native PDC gene. The recombinant yeast are useful in fermentation process for producing lactic acid.

Methods and materials for the production of L-lactic acid in yeast

Growing community of inventors

Maple Grove, MN, United States of America

Pirkko Suominen