Smithkline Beecham Corporation

Other

Janssen Biotech, Inc.

Centocor, Inc.

Ping Tsui

Nabil A Elshourbagy

Pamela A Lane

Raymond W Sweet

Stephanie Van Horn

Ganesh Madhusudan Sathe

Catherine E Ellis

Robert S Ames

Henry M Sarau

James J Foley

Jonathan K Chambers

Laura R Fitzgerald

David M Knight

Jinquan Luo

Juan C Almagro

John Wheeler

Mark Tornetta

Brian Whitaker

Jin Lu

Lisa Vawter

Anuk Das

Qiang Chen

Michael Bardroff

Ramachandra Reddy

Lionella Borozdina-Birch

Lei Shi

Described and claimed herein are combinatorial synthetic Fab libraries displayed on a phage pIX protein. The libraries were built on scaffolds representing the most frequently used genes in human antibodies, which were diversified to mirror the variability of natural antibodies. After selection using a diverse panel of proteins, numerous specific and high-affinity Fabs were isolated. By a process called in-line maturation the affinity of some antibodies was improved up to one hundred-fold yielding low pM binders suitable for in vivo use. This work thus demonstrates the feasibility of displaying complex Fab libraries as pIX-fusion proteins for antibody discovery and lays the foundations for studies on the structure-function relationship of antibodies.

[object Object]

The present invention relates to at least one novel anti-MCP-1 antibody, including isolated nucleic acids that encode at least one anti-MCP-1 antibody, MCP-1, vectors, host cells, transgenic animals or plants, and methods of making and using thereof, including therapeutic compositions, methods and devices.

Anti-MCP-1 antibodies, compositions, methods and uses

Human histamine H3 gene variant-2 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing Human histamine H3 gene variant-2 polypeptides and polynucleotides in diagnostic assays.

Human histamine H3 gene variant-2

The EDG3sb polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing EDG3sb polypeptides and polynucleotides in therapy, and diagnostic assays for such.

Human EDG3sb gene

HOFNH30 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing HOFNH30 polypeptides and polynucleotides in terapy, and diagnostic assays for such.

Human G protein coupled lysophosphatidic acid receptor

EDG1 (mouse) polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing EDG1 (mouse) polypeptides and polynucleotides in therapy, and diagnostic assays for such.

Mouse EDG1

The mouse GPR43 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing mouse GPR43 polypeptides and polynucleotides in therapy, and diagnostic assays for such.

Polynucleotide encoding a Mouse 7-transmembrane GPR43 receptor

Bombesin receptor subtype-3sb polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing Bombesin receptor subtype-3sb polypeptides and polynucleotides in therapy, and diagnostic assays for such.

Human bombesin receptor subtype-3sb

GPR25 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing GPR25 polypeptides and polynucleotides in therapy, and diagnostic assays for such.

GPR 25 polynucleotides

Growing community of inventors

Berwyn, PA, United States of America

Ping Tsui