Other

Ciba-geigy Corporation

Bio-rad Laboratories, Inc.

Gruppo Lepetit

Righetti, Pier Giorgio

Pier Giorgio Righetti

Egisto Boschetti

Pierre Girot

Marcella Chiari

Daniel M Faupel

Attilio Citterio

Ernesto Riva

Luigi Franco Zerilli

Alessandra Maria Bossi

Cecilia Gelfi

Roberto Sebastiano

Frederic Fortis

Materials, apparatuses, methods, and systems for isolating and identifying mixture components on the basis of isoelectric point ('pI'). In one aspect, the materials are particles adapted to have a predetermined isoelectric point. The particles include a porous body into which a plurality of cavities extend. A substance of a predetermined isoelectric point is deposited in the cavities to provide thereby the particle's isoelectric value.

Materials, methods and systems for separating and identifying proteins from mixtures

The present invention refers to the use of novel molecules able to bind tenaciously to silica, borosilicate and silicate surfaces, and thus to modify their properties and characteristics. When applied to silica-based chromatography, it offers important advantages in all cases in which it is necessary to modulate the interaction of analytes with the stationary phase. In capillary zone electrophoresis (CZE), such compounds will eliminate or invert the electroendoosmostic (EEO) flow, greatly simplifying the analysis of negatively-charged compounds and permitting the analysis of bio(macro)molecules via the direct use of naked capillaries.

Methods for surface modification of silica for use in capillary zone electrophoresis and chromatography

Purification of dalbaheptide antibiotics by isoelectric focusing

Immobilized enzyme reactor

Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography are disclosed. The mixed-bed matrices are formed by the copolymerization of a substituted agarose mixture with acrylamide monomers. The disclosed matrices have improved polymerization properties enabling the formation of gels which have higher porosity and elasticity than conventional gels and which are therefore particularly suited to the separation of intermediate size DNA fragments and high molecular mass proteins.

Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for

Formulations for polyacrylamide matrices in electrokinetic and

Described is an isoelectric focusing electrophoretic process for the separation and purification of an amphoteric or neutral chemical compound from one or more electrically charged chemical compound(s). Said process is illustrated below at the example of the purification of a protein from contaminating proteins and salts. It may be carried out in an apparatus especially designed therefor, e.g. an apparatus as depicted in FIG. 1. Said apparatus and various modifications thereof are also claimed. The mixture to be separated is present within a hydraulic flow in chamber 8. Cylinders 5 and 12 contain immobilized pH-gradients or are replaced by amphoteric isoelectric pH-membranes. Each of said pH-gradients and pH-membranes has conductivity and both buffering and titrant capacity in its pH-interval. The extremities of said gradients or pH-membranes forming the ceiling and the floor of chamber 8 have isoelectric points equal to or just higher and just lower than the isoelectric point of the protein of interest which is kept at its isoelectric point in the hydraulic flow and does not enter said pH-gradients and pH-membranes. Contrary thereto the contaminating proteins and salts are driven by an electric field into said pH-gradients or via said pH-gradients or pH-membranes into the electrolyte reservoirs 3 and 14. The described process has the advantage that the desired compound need not be detected and extracted from any matrix, e.g. from said pH-gradients, and that the recovery and purity of the desired compound is higher.

Isoelectric focusing apparatus

Described is an isoelectric focusing electrophoretic process for the separation and purification of an amphoteric or neutral chemical compound from one or more electrically charged chemical compound(s). Said process is illustrated below at the example of the purification of a protein from contaminating proteins and salts. It may be carried out in an apparatus especially designed therefor, e.g. an apparatus as depicted in FIG. 1. Said apparatus and various modifications thereof are also claimed. The mixture to be separated is present within a hydraulic flow in chamber 8. Cylinders 5 and 12 contain immobilized pH-gradients or are replaced by amphoteric isoelectric pH-membranes. Each of said pH-gradients and pH-membranes has conductivity and both buffering and titrant capacity in its pH-interval. The extremities of said graidents or pH-membranes forming the ceiling and the floor of chamber 8 have isoelectric points equal to or just higher and just lower than the isoelectric point of the protein of interest which is kept at its ioselectric point in the hydraulic flow and does not enter said pH-gradients and pH-membranes. Contrary thereto the contaminating proteins and salts are driven by an electric field into said pH-gradients or via said pH-gradients or pH-membranes into the electrolyte reservoirs 3 and 14. The described process has the advantage that the desired compound need not be detected and extracted from any matrix, e.g. from said pH-gradients, and that the recovery and purity of the desired compound is higher.

Isoelectric focusing process and a means for carrying out said process

Growing community of inventors

Milan, Italy

Pier Giorgio Righetti