University of Washington

Children's Hospital Medical Center

Washington State University

Seattle Children's Hospital

University Research Foundation

Binax, Inc.

Phillip I Tarr

Sima S Bilge

James C Vary, Jr

Thomas E Besser

Norman James Moore

The present invention relates generally to the field of microbiology and food sciences. More particularly, the inventor has discovered several polynucleotide sequences encoding the gnd gene and corresponding 6-phosphogluconate dehydrogenase (6-PGD) proteins from different strains ofand polymorphic sequences therein. Novel biotechnological tools, diagnostics, and food screening techniques are provided.

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A method for determining and diagnosing inflammatory enteric disease using an immunochromatographic test device having a multiplicity of test zones. The method tests for the presence of at least one enteric pathogen and at least one of certain inflammatory enteric disease markers. The enteric pathogens tested for can be any number of enteric pathogens such as the pathogens O157, Campylobacter, Salmonella, Listeria, Shigella, and Yersinia. The inflammatory enteric disease markers tested for are fecal lactoferrin, a bacteria marker, a virus marker, and a protozoa marker. Positive results for any one of the pathogens indicates that pathogen as the cause of the inflammatory enteric disease. Positive results for fecal lactoferrin indicate an inflammatory condition of the intestines. Positive results for the bacteria, virus, protozoa markers indicate respectively a bacterial, viral, or protozoan cause of infection as the cause of the disease. Liquid fecal sample is mixed with a mixture containing antibodies to the pathogens and the markers. These antibodies are conjugated to a label. Each test zone on the test device has immovably fixed upon it a complementary antibody that binds specifically with one of the labeled antibodies in the mixture. Positive results appear as a stripe across the corresponding test zone.

Method for detecting enteric disease

Polypeptides encoded by a continuous segment of chromosomal DNA from E. coli O157:H7, isolated on plasmid pSC(overlap) (ATCC No. 69648), that encodes an adhesin (SEQ ID NO:5) that mediates bacterial colonization of bovine intestines, vaccines derived therefrom, and antibodies directed against the adhesin.

Escherichia coli O157:H7 epithelial adhesion and vaccine

Escherichia coli O157:H7 epithelial adhesin

An isolated nucleic acid molecule that hybridizes under stringent conditions to SEQ ID NO:1 or its complement and to the DNA of enterohemorrhagic E. coli O157:H7 but not to the DNA of enteropathogenic E. coli O55:H7.

Nucleic acid probes for detecting E. coli O157:H7

Growing community of inventors

Seattle, WA, United States of America

Phillip I Tarr