Martek Biosciences Corporation

Philip Edward Coughlin

Jonathan Miles Brown

Frank Elbert Anderson, Iii

Steven William Homans

Charles Tobias Weller

Carrie Ann Zimmerman-Wilson

Eddie James Oliver, Jr

Eddie James Oliver

Frank Elbert Anderson

The invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.

13C, 15N, 2H labeled proteins for NMR structure determinations and their preparation

This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.

13C,15N ,2H labeled proteins for NMR structure determinations and their preparation

Peptidic molecules suitable for three dimensional NMR structure determination are provided which have been substituted with .sup.13 C and/or .sup.15 N in the backbone and/or at the .alpha.-carbon with .sup.2 H but not otherwise in the sidechains of the amino acid residues. One or more amino acids, amino acid species or substantially all of the amino acid residues of the molecule may be so substituted. A method of making the substituted peptidic molecules substituted in this manner is provided.

Peptidic molecules which have been isotopically substituted with .sup.13

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Wilmington, DE, United States of America

Philip Edward Coughlin