Dsm IP Assets B.v.

Dsm Nutritional Products Ag

Paul Frederick Mugford

Martin Schurmann

Monika Mueller

Jaroslav A Kralovec

Weijie Wang

Alfred Rolle

Theophilus Cornelus Van Den Heuvel

Disclosed herein are host cells expressing a polypeptide, wherein the polypeptide hydrolyzes an ester linkage of a triacylglycerol in an oil comprising at least one long-chain polyunsaturated fatty acid, methods for using such host cells, and processes for production of a lipase using such host cells.

Partial enzymatic hydrolysis of triacylglycerols to produce long-chain polyunsaturated fatty acid

A method for producing diglycerides is provided. The method includes combining (i) an oil comprising at least one polyunsaturated fatty acid in the form of ethyl esters, free fatty acids, and/or combinations thereof, (ii) lipase, and (iii) glycerol in water to produce diglycerides with a high level of purity. Highly pure diglycerides obtained according to the method are also provided.

Process for the production of diglycerides

Disclosed herein are host cells transformed with a nucleic acid molecule comprising a polynucleotide sequence, wherein the polynucleotide sequence encodes a polypeptide that hydrolyzes an ester linkage of a triacylglycerol in an oil comprising at least one long-chain polyunsaturated fatty acid, methods for using such host cells, and processes for production of a lipase using such host cells.

Partial enzymatic hydrolysis of triacylglycerols

Disclosed are methods of recovering urea from a urea/oil complex by drying the complex, combining the complex with water to form a urea solution, and removing water from the urea solution. Methods for recycling the urea in urea complexation processes are also disclosed. Also disclosed are methods of recovering polyunsaturated fatty acids or derivatives thereof from the urea/oil complexes.

Growing community of inventors

Halifax, Canada

Paul Frederick Mugford