Vertex Pharmaceuticals, Inc.

Invitrogen Corporation

Paul A Negulescu

Alec Tate Harootunian

James Edward Sinclair

Ashvani Singh

Minh Vuong

Philip Emanuel Salzmann

Javier Humberto Flores

Fred F VanGoor

Roger Y Tsien

Gregor Zlokarnik

Tom Knapp

Tim Rink

A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an 'Ussing chamber.'

Multiwell assay apparatus and method for determining biological activity of compounds

Multiwell plate assembly for use in high throughput assays

The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.

Photon reducing agents for fluorescence assays

Growing community of inventors

Del Mar, CA, United States of America

Paul A Negulescu