Gist-brocades N.v.

Plant Genetic Systems N.v.

Plant Genetic Systems

Genencor International, Inc.

Patrick Stanssens

Wilhelmus J Quax

Ignace Lasters

Adrianus Wilhelmus Vollebregt

Nadir Mrabet

Yves Laroche

Shoshana Wodak

Gaston Matthyssens

Mutant microbial .alpha.-amylases with increased thermal, acid and/or

The invention pertains to a method for the production of a biologically active modified protein derived from a starting protein having essentially the same kind of biological activity with an attendant modulation effect on, particularly increase of, the stability as compared with that of the starting protein. The method comprises substituting an arginine residue for a lysine residue of the starting protein at a site that can sterically accommodate the substitution, without substantially altering the biological activity of the starting protein, said site being preferably of low solvent accessibility, at interfaces between domains or sub-units of the starting protein.

Methods and means for controlling the stability of proteins

Growing community of inventors

St. Denijs Westrem, Belgium

Patrick Stanssens