Dow Agrosciences LLC

Other

Pioneer Hi-bred International, Inc.

Curagen Corporation

Otto Folkerts

Oswald R Crasta

Jacob T Gilliam

Joyce R Maddox

Jon Duvick

Jonathan P Duvick

Katherine Armstrong

Kelley Ann Smith

Donald J Merlo

Aaron Todd Woosley

Dayakar Reddy Pareddy

Scott A Young

Beth C Rubin-Wilson

Nicole Linda Hopkins

Timothy Denver Hey

Joseph F Petolino

Aragula Gururaj Rao

Lining Guo

Michael A Menke

Michael Ainley

Scott Belmar

James A McSwiggen

Carl R Simmons

James A Strickland

Neil Madison Cowen

Virginia C Crane

James K Petell

James M Hasler

Kitisri Sukhapinda

Yogesh Kumar Sharma

Thomas Skokut

Patricia Ann Owens Merlo

Michael G Zwick

Tom Skokut

Brent E Edington

Kelley A Smith

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated fromand. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use

The present invention provides transformed plants comprising a polynucleotide enconding amino polyol amine oxidase (APAO ) fromand a method of expressing APAO transgenic plants and seeds. In this way, transgenic plant can be produced having fumonisin degrading capability, as well as with the capability of producing the degrading enzymes.

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from . Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use

Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.

Compositions and methods for fumonisin detoxification

The maize myo-inositol-1-phosphate synthase (MIP synthase) promoter is a novel embryo specific regulatory sequence useful in expressing heterologous nucleic acids in plants.

Maize MIP synthase promoter

Monoclonal antibodies expressed in plant cells bind targeted transit peptides to decrease steady state levels of passenger proteins in plant organelles.

Antibody-mediated down-regulation of plant proteins

Regulatory sequences derived from the maize per5 gene have utility in plant biotechnology.

Regulatory sequences for transgenic plants

The invention relates to the genetic manipulation of plants, particularly to the expression of genes involved in oxylipin metabolism in plants. Nucleotide sequences encoding homologues of Old Yellow Enzyme, and inducible promoters and proteins thereof, are provided. The sequences find use in modifying oxylipin metabolism in plants, increasing the resistance of plants to stress, regulating gene expression in plants, and in the production of oxylipins in plants.

Polynucleotides encoding monocot 12-oxo-phytodienoate reductases and methods of use

Genes-encoding a delta-9 CoA desaturase from have been isolated. The proteins encoded by genes, when expressed in a plant, can alter the saturate levels of the oil.

Modification of fatty acid composition in plants by expression of a fungal acyl-CoA desaturase

Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding &bgr;-glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.

Use of &bgr;-glucosidase to enhance disease resistance and resistance to insects in crop plants

This invention relates to compositions and methods useful in the production of transgenic plants. In particular, the invention relates to maize metallothionein gene transcription regulatory sequences which are useful for directing expression of heterologous DNAs in plant embryo tissue. The invention also relates to expression cassettes containing the promoter sequences and to transgenic plants containing the expression cassettes.

Regulatory sequences useful for gene expression in plant embryo tissue

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Guilford, CT, United States of America

Otto Folkerts