Danisco US Inc.

Genencor International, Inc.

Oreste J Lantero, Jr

Jayarama K Shetty

Nigel S Dunn-Coleman

Suzanne E Lantz

Michael Jay Pepsin

Gopal K Chotani

Benjamin S Bower

Toby M Baldwin

Bruce A Strohm

Suzanne Breneman

Bradley A Paulson

Described are compositions and methods relating to the use of a glucoamylase in combination with a phytase in starch processing to reduce the levels of phytic acid in end-products.

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The present invention relates to filamentous fungal host cells and particularlyhost cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.

Enzyme compositions comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease

Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates

The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25-40&#xb0; C. to obtain a fermentation broth having 5 to 20% ethanol.

Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis

The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25&#x2013;40&#xb0; C. to obtain a fermentation broth having 5 to 20% ethanol.

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Belvidere, IL, United States of America

Oreste J Lantero, Jr