Nestec S.a.

Olivier Mignot

Florence Rochat

Eduardo Schiffrin

Jean-Richard Neeser

Beat Mollet

Dominique Brassart

Herbert Hottinger

Alain Servin

Harriet Link

Anne Donnet

Jacques Edouard Germond

Koichiro Tsuda

Lactobacillus johnsonii strain CNCM I-1225 adheres to Caco-2 cells and inhibits adhesion thereto by enterovirulent and enteroinvasive pathogens.

Lactobacillus johnsonii CNCM I-1225

The present invention concerns Lactobacillus bulgaricus wherein the lactate dehydrogenase activity is lower than 50%, preferably lower than 10%, of the lactate dehydrogenase activity of Lactobacillus bulgaricus wild type strain. The present invention concerns also the food composition comprising said Lactobacillus bulgaricus.

Lactobacillus bulgaricus having decreased acid production and/or

Bifidobacterium breve CNCM I-1226, Bifidobacterium infantis CNCM I-227 and Bifidobacterium longum CNCM I-1228 adhere to and competitively exclude pathogenic bacteria from Caco-2 cells. The strains may be incorporated into yogurts, acidified milks and other food compositions.

Bifidobacteria

Yogurt is prepared by inoculating and fermenting a milk with a strain of Streptococcus thermophilus and a lac(-) mutant strain of Lactobacillus bulgaricus, the DNA of which has a deletion of at least part of the .beta.-galactosidase gene.

Preparation of yogurt with lac(-) L. bulgaricus

Growing community of inventors

Blonay, Switzerland

Olivier Mignot