Toyota Jidosha Kabushiki Kaisha

Kabushiki Kaisha Toyota Chuo Kenkyusho

Nobuki Tada

Toru Onishi

Junji Ito

Nobuhiro Ishida

Rie Hirao

Satoshi Katahira

Noriko Yasutani

Risa Nagura

Kenro Tokuhiro

Chie Imamura

Hibiki Matsushita

Takashi Shimamura

Toshiaki Kimura

A stable transformant, in which a gene of interest is incorporated into the genome, is simply and efficiently produced. A method for producing a transformant, comprising a step of introducing into a host, linear genome-introduced nucleic acid fragment(s) comprising a gene of interest and a helper plasmid for transformation having a pair of homologous recombination sequences for incorporation of the linear genome-introduced nucleic acid fragment(s), and then selecting a transformant, in which the gene of interest is incorporated into the predetermined position in the host genome and the gene of interest is expressed therein.

Helper plasmid for transformation, method for producing transformant using the same, and transformation method

This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.

Mutant gene associated with improvement in ethanol productivity via ethanol fermentation and method for producing ethanol using the same

An acetic acid metabolizing ability of a recombinant yeast strain having xylose-metabolizing ability is to be improved. In such a recombinant yeast strain having xylose-metabolizing ability, the acetaldehyde dehydrogenase gene has been introduced and a gene encoding NADH dehydrogenase involved in reoxidation of cytoplasmic NADH on the mitochondrial outer membrane has been suppressed.

Recombinant yeast and a method for producing ethanol using the same

A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.

Protein having xylose isomerase activity and use of same

The invention is intended to improve xylose assimilation ability and ethanol fermentation ability in a xylose-assimilating yeast into which a xylose isomerase gene has been introduced. The amount of NADH produced by the recombinant yeast into which the xylose isomerase gene had been introduced as a result of the enzymatic reaction of acetohydroxy acid reductoisomerase is lowered.

Recombinant yeast and method for producing ethanol using the same

Thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which are the offensive odor components, are provided. Disclosed are thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which belong to

Microorganism and deodorizer containing the same

The present invention provides, inter alia, an improvement in the production of a desired product from yeast, wherein the growth rate and fermentation rate of the yeast are maintained at excellent levels. The present invention also provides, inter alia, for the introduction of a foreign gene into a yeast host cell, wherein the foreign gene encodes an enzyme involved in the production of a desired product. The yeast may also constitutively express a HAP4 gene, or a homologous gene thereof, to significantly improve the ability of the yeast to produce the desired product while maintaining its growth rate and fermentation rate. In some embodiments, a yeast mutant is used which is a mutant strain having lowered alcohol productivity vis-&#xe0;-vis a wild-type strain.

Growing community of inventors

Nisshin, Japan

Nobuki Tada