Ajinomoto Co., Ltd.

Natalia Pavlovna Zakataeva

Irina Lyvovna Tokhmakova

Vladimir Veniaminovich Aleshin

Kazuo Nakanishi

Vitaliy Arkadievich Livshits

Petr Vladimirovich Troshin

Vitaly Arkadievich Livshits

Ekaterina Aleksandrovna Kutukova

Elvira Borisovna Voroshilova

Yury Ivanovich Kozlov

Natalia Viktorovna Stoynova

Sergei Vladimirovich Mashko

Alla Valentinovna Belareva

Vitaliy Arkadyevich Livshits

Evgeniya Aleksandrovna Malykh

Anna Evgenievna Novikova

Sergey Viktorovich Gronsky

Maria Kharchenko

Aleksander Krylov

Ekaterina Andrianova

Mikhail Baboshin

Lyubov Golubeva

Ekaterina Kovaleva

Mikhail Shupletsov

Kazuo c/o Ajinomoto Co Inc Nakanishi

The present invention provides a method for producing a target substance, the biosynthetic pathway of which is ATP-dependent, for example, amino acids, nucleosides, nucleotides, isoprenoids, and peptides, by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having H-translocating membrane-bound pyrophosphatase activity, for example, the hppA gene native toor a variant thereof.

Method for producing target substance by bacterial fermentation

The present invention describes a bacterium which has an ability to produce an amino acid and in which the rhtC gene encoding a protein having an enhanced activity of imparting L-threonine resistance to a bacterium expressing the protein. Preferably, the bacterium further includes an rhtB gene encoding for a protein having an enhanced activity of imparting to a bacterium L-homoserine resistance expressing the protein. The present invention also describes a method of cultivating the bacterium in a culture medium to produce and accumulate amino acids in the medium, and the amino acid is recovered from the medium.

Method for producing an amino acid using a bacterium overexpressing an rhtC gene

Methods for producing purine nucleosides, and purine nucleotides, such as inosine and 5'-inosinic acid are provided which include using a bacterium belonging to the genusor to the genuswherein the purine nucleoside productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. Also disclosed is the amino acid sequence of the YdhL protein fromand the gene encoding it.

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A method is provided for producing a purine nucleoside, such as inosine and guanosine, and a method for producing a 5'-purine nucleotide such as 5&#x2032;-inosinic acid or 5&#x2032;-guanylic acid, using a bacterium belonging to the either genusor genus, wherein purine nucleoside productivity of said bacterium is enhanced by enhancing an activity of a protein encoded by the yeaS (leuE) gene.

A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of the yfiK gene is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of the yggA gene is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genuswhich has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of an L-amino acid excretion protein is described. A method for producing the L-amino acid using the bacterium is also described.

A bacterium belonging to the genusand having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.

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Moscow, Russia

Natalia Pavlovna Zakataeva