Bizen Chemical Co., Ltd.

Iwate Prefectural Government

Naomichi Bamba

Yoshihisa Misawa

Yoshio Shimizu

Hiroshi Tabata

Tadahiro Tsushima

Mitsumasa Mankura

Akira Yano

Hidetoshi Yamada

Sayaka Yuki

The present invention provides a lipoxygenase that enables the highly efficient formation of an oxide of a highly unsaturated fatty acid or an oxide of a highly unsaturated fatty acid derivative from a highly unsaturated fatty acid or a highly unsaturated fatty acid derivative. The present invention provides a lipoxygenase that enables the highly efficient formation of 8-hydroxyeicosatetraenoic acid from arachidonic acid, the highly efficient formation of 8-hydroxyeicosapentaenoic acid from eicosapentaenoic acid, and the highly efficient formation of 10-hydroxy docosahexaenoic acid from docosahexaenoic acid. Specifically, the present invention provides a polynucleotide that comprises the nucleic acid sequence represented by SEQ ID NO: 1 and 3, or a homolog thereof, and a polypeptide that comprises the amino acid sequence represented by SEQ ID NO: 2 and 4, or a homolog thereof. Also provided is a composition that comprises a membrane protein of an organism belonging to the class Malacostraca and is for carrying out a lipoxygenase reaction.

Oxidation of highly unsaturated fatty acids with novel 8-lipoxygenase derived from Malacostraca

A method of chemically extending a carbon chain of an unsaturated fatty acid for conversion into a different unsaturated fatty acid has been reported. The present invention shortens reaction steps of conventional methods, and completes a carbon chain extending reaction in a shorter time. The present invention provides a method of extending a carbon chain of an unsaturated fatty acid by two carbons, comprising steps of four stages including a short-path conversion reaction of an unsaturated fatty chain obtained from an unsaturated fatty acid into a malonic ester derivative, and heating of the malonic ester derivative to reflux in a lower fatty acid solution. The method of the present invention can complete a carbon chain extending reaction in a shorter time.

Synthesis of long-chain unsaturated fatty acid by chemical reaction of carbon chain extension

The present invention relates to a method for the chemical conversion of an unsaturated fatty acid, particularly a carbon chain extension reaction. According to the present invention, a method for extending the length of a carbon chain in an unsaturated fatty acid by two carbon atoms is provided, said method comprising a step of heating a malonic acid ester derivative of an unsaturated fatty acid to reflux in a lower fatty acid solution in the presence of an antioxidative agent. It is preferred that the unsaturated fatty acid is an unsaturated fatty acid having 16 to 24 carbon atoms. It is preferred that the unsaturated fatty acid is selected from the group consisting of linoleic acid, linolenic acid, arachidonic acid, stearidonic acid, icosatetraenoic acid, icosapentaenoic acid, tetracosahexaenoic acid and docosahexaenoic acid. According to the method of the present invention, a carbon chain extension reaction can be completed within a shorter time.

Method for chemical conversion of unsaturated fatty acid by carbon chain extension reaction

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Akaiwa, Japan

Naomichi Bamba