Mitsui Chemicals, Inc.

Naokazu Naitoh

Daisuke Mochizuki

Masaru Honjo

Hiroshi Uchida

Kazuya Matsumoto

A DNA encoding 20K hGH is connected directly to a gene encoding OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, is transformed by the plasmid and cells of the resulting transformant strain are cultured for secretory production of the 20K hGH in the periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the periplasm is low.

Method for secretory production of human growth hormone

A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.

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Chiba, Japan

Naokazu Naitoh