Monell Chemical Senses Center

Cargill Inc.

Nancy Ellen Rawson

Mehmet Hakan Ozdener

John Thomas McDonald, Jr

Brian Guthrie

Sean S Smith

Chin Hong Paul Kim

Brian B Anderson

Allen R Muroski

Var Len St Jeor

John H Teeter

Karen K Yee

The invention provides methods of culturing mammalian taste cells, including taste receptor cells. Cells are maintained for a duration of up to three months and longer while maintaining molecular and functional characteristics of mature taste cells. The cells are cultured on coated cell culture vessels and, from first replacement of medium onwards, the medium is replaced in intervals of at least 5 days. The invention further provides isolation and culturing methods of taste cells wherein the time that the cells are exposed to isolation solution and proteolytic enzymes is minimized and the cells are cultured in coated culture vessels with the medium replaced in intervals of at least 5 days from first replacement onwards. The invention further provides cultured taste cells, transfection and assay methods, and taste cell assay buffers with an osmolarity of about 300-320 and pH of about 7.0-7.3.

Methods for culturing mammalian taste cells

Method for culturing mammalian taste cells

The present invention provides a method for determining cellular response to stimuli. The cells to be tested, for example, may be contained in a section of taste-bud containing lingual epithelium.

Methods for determining cellular response to stimuli

The present invention provides methods of treating complicated anosmia in a mammal, methods of enhancing the rate of olfactory nerve recovery in a mammal, and methods of enhancing nerve regeneration in a mammal by administering an effective amount of a retinoid compound to the mammal.

Methods of treating anosmia and repopulating olfactory nerves with retinoids

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Drexel Hill, PA, United States of America

Nancy Ellen Rawson