Phillips Petroleum Company

Research Corporation Technologies, Inc.

Motohiro Fuke

Kotikanyadan Sreekrishna

Rica H Potenz

W Richard Mccombie

Ricky N Wong

Robert D Barr

Farhad Marashi

Karen M Vineyard

Frances M Davis

Kathryn Parker

Novel DNA constructs comprising yeast regulatory regions plus the structural coding region for human tumor necrosis factor, are disclosed. These novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used for yeast transformation and ultimately for the production of human tumor necrosis factor by yeast.

Yeast production of human tumor necrosis factor

Yeast strains are transformed with, and selected for, using DNA fragments encoding a gene function not present in the wild-type yeast strains. The invention requires transforming the yeast with DNA fragments which comprise the gene function which the wild-type yeast strain is lacking; regeneration of the transformants on a non-selective regeneration medium; then selection of those colonies which are capable of growth on a carbon and energy source which requires expression of the gene function provided by the DNA fragment. Thus, transformation with such DNA fragments allows for positive selection of transformed strains.

Carbon and energy source markers for transformation of strains of the

Growing community of inventors

Tulsa, OK, United States of America

Motohiro Fuke