Upfront Chromatography A/s

Upfront Chromatography

Morten Aae Olander

Marie Bendix Hansen

Allan Otto Fog Lihme

Timothy J Hobley

Allan Otto Lihme

Inga Vaarst Andersen

Jurgen Hubbuch

Owen R T Thomas

Marcos Simon

Irini Theodossiou

Owen Robert Tyrynis Thomas

Inga Vaarts Andersen

Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 &#x3bc;m; c) optionally washing the column; d) eluting at least one protein from the adsorbent.

Fractionation of protein containing mixtures

Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, the method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying the mixture to an adsorption column comprising an adsorbent, the adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 &#x3bc;m; c) optionally washing the column; d) eluting at least one protein from the adsorbent.

The invention relates to a method of distributing a liquid in the fluid bed of an up-flow or a down-flow fluid bed reactor. The invention provides efficient distribution and plug flow like fluid flow through the fluid bed where turbulence and/or back-mixing of the fluid are minimized. In accordance with the invention a fluid bed system for use in treating a fluid by contacting the fluid with a solid phase media is provided and the system includes a reactor chamber adapted to contain the solid phase media and at least one fluid distribution means adapted to distribute and/or deliver the fluid to be treated among the particles of the medium.

Bed adsorption system

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 &mgr;m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

Particulate material for purification of bio-macromolecules

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Copenhagen, Denmark

Morten Aae Olander