University of California

Mohammad Aghaamoo

Abraham Phillip Lee

Xuan Li

Neha Garg

Yu-Hsi Chen

Michelle A Digman

Gopakumar Kamalakshakurup

Francesco Palomba

Gisela Lin

Xuhao Luo

Marzieh Ataei

The present invention features the use of cavity acoustic transducers (CATs) to apply mechanical stimuli on cells. CATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear on them. The present invention may use such a portable, automated, and high throughput device for cell transfection.

Cavity acoustic transducer (CAT) for shear-induced cell transfection

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting

The present invention features the use of lateral cavity acoustic transducers (LCATs) to apply mechanical stimuli on cells. LCATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear-induced cell deformation on them. The present invention may use such a portable, automated, and high throughput device for shear-induced cell transfection.

Lateral cavity acoustic transducer (LCAT) for shear-induced cell transfection

Growing community of inventors

Irvine, CA, United States of America

Mohammad Aghaamoo