Case Western Reserve University

Miklos Gratzl

Sumitha Nair

George Rozakis

Christian A Zorman

Punkaj Ahuja

Maria A Peshkova

Koji Tohida

Kwong Yue Hui

Andrea Cserey

John W Stanton

Zhehao Zhang

Tamas Cseefalvi

Koji Tohda

Jian Yang

Gautam Nithyanand Shetty

Armand Krikorian

Disha Sheth

Maria Peshkova

A microfluidic pH-stat with a specially-is designed slide and portable device can be used for point-of-care enzyme diagnostics. The slide includes a microchamber and a substrate for the enzyme being tested. The substrate is homogenized with the sample in the microchamber to form a test volume. The microchamber includes a working microelectrode that injects current to split water in the test volume to generate hydrogen ions and/or hydroxide ions and a micro-pH-electrode to measure a pH of the test volume; the slide also includes a reference microelectrode. The device includes a processor to adjust the injected current based on the pH of the test volume and determine an activity of the enzyme based on an amount the injected current is adjusted.

Microfluidic pH-stat for point of care (POC) enzyme diagnostics

A system for multiparametric analysis includes a substrate and a three-dimensional (3D) cell aggregate. The substrate has a major surface and includes at least one radial electrode array. The 3D cell aggregate is disposed on the major surface of the substrate. The 3D cell aggregate has a longitudinal surface at least a portion of which covers one or more of the electrodes of the radial electrode array.

Multicellular system and method for multiparametric analysis

An in vitro sensor for point-of-care detection of at least one analyte or reaction product includes an inert, impermeable substrate, a sensing system, and a reference system. The substrate includes a first transparent surface oppositely disposed from a second surface and first and second cavities. Each of the first and second cavities defines an opening at the second surface. The sensing system is disposed in at least a portion of the first cavity and includes an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of non-transparent microbeads associated with at least one of the analyte-detection optode membrane and the analyte-permeable membrane. The analyte-permeable membrane is layered upon the analyte-detection optode membrane and covers the opening of the first cavity. The reference system is disposed in at least a portion of the second cavity.

In vitro point-of-care sensor and method of use

A device for adjustment of the pH of a target liquid includes a working electrode (), an electrolyte chamber () which holds an electrolyte (), a counter electrode () in electrical contact with the electrolyte, a junction () which spaces the electrolyte from a target liquid () when the working electrode is in contact therewith, and a source of current (), for supplying current to the working electrode for electrolysis of water at the working electrode, whereby the pH of the target solution is adjusted.

Device for the adjustment of the PH of aqueous solutions

A sensor probe suited for implanting into the skin of a person includes a sensor body which may be formed from a polymer which includes 2-hydroxyethyl methacrylate (HEMA). A sensing system is supported by the body. The sensing system exhibits a detectable change when the probe is exposed to the analyte in the fluid. The sensing system may include an enzyme capable of catalyzing a reaction of the analyte to form a reaction product and a dye system which absorbs in the infrared region of the spectrum in response to the reaction product.

Sensor system

A burette () suitable for delivery of a reagent into a target solution () employs diffusion for delivering the reagent. The reagent is in the form of a solution, which is combined with a matrix material (), such as a gel or porous ceramic. A membrane () covers a delivery outlet () to the burette. In one embodiment, the delivery outlet comprises a plurality of fine bores (), each one filled with or covered by a membrane (). Stirring of the burette or target solution is achieved with a stirring means (). A heating or cooling means () heats a tip () of the burette.

Device for precise chemical delivery and solution preparation

A method for solving deconvolution problems where it is desired to reconstruct a signal over a time range or another variable of interest involves comparing shapes of measured and reconstructed plots. The optimization method is based on minimizing the error in shape (as opposed to the square errors in amplitude). A shape approach method characterizes similarity of two functions by computing the angle between the two when they are treated as two vectors in the n dimensional space where n is the number of data points it is desired to consider from both functions (the functions themselves may consist of more than n data points). A new approximation is then created by trying to decrease the disimilarity between the actual and predicted functions. This dissimilarity is measured as the angle between the two corresponding vectors, so the measure of dissimilarity is the size of the angle. A much closer matching between the reconstructed plot and what may be expected in practice is achieved with this method than is conventionally achieved with least squares or Fourier transform methods.

Growing community of inventors

Mayfield Heights, OH, United States of America

Miklos Gratzl