California Institute of Technology

Centre National De La Recherche Scientifique

Ecole Superieure De Physique Et De Chimie Industrielles De La Ville De Paris

Inserm (institut National De La Sante Et De La Recherche Medicale)

Mikhail G Shapiro

Arash Farhadi

Azita Emami

Raymond W Bourdeau

Anupama Lakshmanan

Saransh Sharma

David Maresca

Daniel P Sawyer

Audrey Lee-Gosselin

Dan I Piraner

Suchita P Nety

Avinoam D Bar-Zion

Mohamad H Abedi

Abhinav Agarwal

Brittany Moser

Jerzy O Szablowski

Manuel Alejandro Monge Osorio

Atousa Nourmahnad

Gabrielle H Ho

Mengtong Duan

Robert C Hurt

Morteza Gharib

Richard A Andersen

Mickaël Tanter

Michael Ortiz

Stefanie Heyden

Charlie Demene

George J Lu

Sumner L Norman

Vasileios Christopoulos

David Reza Mittelstein

Justin Lee

Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

Gas-filled structures and related compositions, methods and systems to image a target site

Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.

Thermal control of T-cell immunotherapy through molecular and physical actuation

Methods and systems are provided for decoding movement intentions using functional ultrasound (fUS) imaging of the brain. In one example, decoding movement intentions include determining a memory phase of a cognitive state of the brain, the memory phase between a gaze fixation phase and movement execution phase, and determining one or more movement intentions including one or more of intended effector (e.g., hand, eye) and intended direction (e.g., right, left) according to a machine learning algorithm trained to classify one or more movement intentions simultaneously.

Decoding movement intention using ultrasound neuroimaging

Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

Gas vesicle expression systems, gas vesicle constructs and related genetic circuits, vectors, mammalian cells, hosts, compositions, methods and systems

Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

Genetically engineered gas vesicle gene clusters, genetic circuits, vectors, prokaryotic cells, compositions, methods and systems for contrast-enhanced imaging

The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.

Compositions, methods and systems for gas vesicle based cavitation

Provided herein are thermomer monomer, thermomer dimers, thermomer monomeric constructs, thermomer dimeric complexes, and related gene expression cassette vectors, cells, surfaces devices, compositions methods and systems, that provide a thermobioswitch suitable to control location and/or binding of cargo moiety of interest in a temperature regulated manner.

Modular dimerization thermoswitches and related monomers, dimers, constructs, dimeric complexes, vectors, cells, surfaces, devices compositions, methods and systems

Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

Thermal bioswitches and related genetic circuits, vectors, cells, compositions, methods and systems

A method for in-vivo monitoring of a target internal volume of a mammal that includes: (a) placing the target internal volume proximal to a three-dimensional magnetic field generator; (b) generating first, second, and third magnetic field gradients along respective first, second, and third axes that are mutually orthogonal; (c) measuring first, second, and third magnetic fields with a three-dimensional magnetic sensor disposed in an ingestible capsule, the ingestible capsule disposed in the target internal volume; (d) with a controller in electrical communication with the three-dimensional magnetic sensor, generating a magnetic sensor output signal that encodes a measurement of the first, second, and third magnetic fields; (e) broadcasting the magnetic sensor output signal from an antenna disposed in the ingestible capsule, and (f) receiving the magnetic sensor output signal with a receiver. The received magnetic field data can be used to determine the three-dimensional position of the ingestible capsule.

In-vivo monitoring of an internal volume of a mammal using magnetic field gradients

A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.

Surgical alignment by magnetic field gradient localization

The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.

Burst ultrasound reconstruction with signal templates and related methods and systems

An apparatus for producing magnetic field gradients includes (a) a first planar electromagnet coil set configured to produce a first magnetic field gradient with respect to a first axis, (b) a second planar electromagnet coil set configured to produce a second magnetic field gradient with respect to a second axis that is orthogonal to the first axis, (c) a third planar electromagnet coil set configured to produce a third magnetic field gradient with respect to a third axis that is orthogonal to the first and second axes, and (d) a controller configured to selectively provide power to the first, second, and/or third electromagnet coil sets to sequentially produce a localization magnetic field gradient with respect to each of the first, second, and third axes, at least a portion of each localization magnetic field gradient having a monotonically-varying magnetic field magnitude along a respective axis.

Electromagnet gradient coil apparatus for micro-device localization

Growing community of inventors

Los Angeles, CA, United States of America

Mikhail G Shapiro