Bayer Corporation

Michael Zachariou

Charles V Olson

Eva Sajan

Ricaredo Matanguihan

Hyun J Park

Jonathan Mazer

A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.

Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration

A chemiluminescence-based gel assay may be used as an indicator of fermenter health. The assay is performed using a sample of fermentation medium essentially directly from the fermenter (with no need for complicated purification of the sample before performing the assay). Results from the assay may be correlated with the final product purification yield of a given protein/purification protocol system. This may then be used as a predictor of purification yield before actually performing the purification.

Growing community of inventors

Walnut Creek, CA, United States of America

Michael Zachariou