Vogelbusch Gmbh

Tate & Lyle Ingredients Americas LLC

Universitá Degli Studi Di Milano-Bicocca

Syconium Lactic Acid Gmbh

Univeristät Für Bodenkultur Wien

Biopolo S.c.a.r.l.

Lonza Inc.

Michael Sauer

Diethard Mattanovich

Danilo Porro

Hans Marx

Stefan Pfluegl

Brigitte Gasser

Michael Maurer

Paola Branduardi

Martin Dragosits

Magnus Ask

Minoska Valli

Rakesh Koppram

The present invention provides a method for producing lactic acid in a recombinant yeast cell culture using glucose as carbon source comprising a first, seed fermentation stage to produce biomass wherein the yeast is cultivated in a culture medium at a pH of 5 to 7, followed by a second, a production fermentation stage with biomass from the seed fermentation to produce lactic acid, wherein the yeast is cultivated in a culture medium at low p H using a yeast strain that is engineered to have lactate dehydrogenase (LDH) activity and optionally has decreased or knocked-out pyruvate decarboxylase (PDC) activity.

Method for producing lactic acid in recombinant yeast cell culture

The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivatingin the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use ofin one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.

Fermentation process for producing chemicals

The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line&#x2014;to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and&#x2014;to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.

Production cell line

Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is aspp. or aspp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.

Ascorbic acid production from yeast

A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pH)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHabove the gate pH are retained and the cells having a pHbelow the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.

Methods for selecting a yeast population for the production of an organic acid and producing an organic acid

Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is a Zygosaccharomyces spp. or a Kluyveromyces spp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.

Growing community of inventors

Vienna, Austria

Michael Sauer