Centre National De La Recherche Scientifique

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Universitéde Strasbourg

Simon Fraser University

Centre International De Recherche Aux Frontieres De La Chimie

Scientifique Université De Strasbourg

Michael Ryckelynck

Alain Wagner

Andrew David Griffiths

Sylvain Ursuegui

Amir Abdolahzadeh

Shanker Shyam Sundhar Panchapakesan

Alexis Autour

Razvan Cojocaru

Peter Unrau

Adrian Ferré D'Amaré

Elena Dolgosheina

Robert Trachman

Ketty Pernod

Sunny Chiu Yuk Jeng

RNA aptamers are disclosed with distinct fluorescent properties, fluorophore binding affinities, and salt dependence. Also disclosed are corresponding fluorophores, with selected fluorophores evidencing high cellular permeability. The aptamer's high fluorophore affinities, the high brightness of the bound complexes, and their thermal and salt stability, provide distinct aspects of the disclosed aptamers.

Fluorogen-binding RNA aptamers

The present invention relates to a method for capturing a molecular target present in the aqueous phase of a water-in-oil emulsion, said method being based on the use of a binding system comprising (a) a surfactant bearing a functional moiety on its hydrophilic head group and (b) a chemoprobe that acts as a molecular staple between the functionalized surfactant and the molecular target and comprises at least two distinct domains namely (i) at least one capture moiety which is able to specifically bind the molecular target and (ii) at least one binding domain which is able to interact with the functional group of the surfactant through covalent or non-covalent interactions, directly or through a binding intermediary.

Intra-droplet surface engineering to capture a molecular target

Growing community of inventors

Strasbourg, France

Michael Ryckelynck