Roche Innovation Center Copenhagen A/s

Michael Meldgaard

Dennis Jul Hansen

Christoph Rosenbohm

Marianne B Mogensen

Inna Appeldorff Larsen

Marianne Mogensen

The present invention relates to the field of oligonucleotide conjugates and to methods of synthesis thereof. In the present method a low-water content solvent environment allows a more efficient conjugation, reducing the amount of conjugate moiety needed and increasing the conjugation reaction speed.

Oligonucleotide conjugation process

Methods for cleaving oligonucleotides from a solid support are described as are methods for synthesizing an oligonucleotide on a solid support and subsequently cleaving the oligonucleotide from the solid support. In the methods, the 3' nucleoside of the oligonucleotide attached to the solid support is a LNA nucleoside. The method entail treating the bound oligonucleotide with a concentrated ammonium hydroxide solution for about 30 minutes to about 6 hours.

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Hørsholm, Denmark

Michael Meldgaard