Large Scale Biology Corporation

Biosource Technologies, Inc.

Other

Kentucky Bioprocessing, Inc.

Biosource Genetics Corporation

Hps Biotechnologies, Inc.

Michael J McCulloch

Stephen J Garger

R Barry Holtz

Thomas H Turpen

Richard Barry Holtz

Genadie G Sverlow

Terri I Cameron

Michelle L Samonek-Potter

John A Lindbo

Gregory P Pogue

Guy R Della-Cioppa

Jonathan E Lawrence

Cynthia S Gross

Barry Bratcher

Guy Richard della-Cioppa

Stephen John Garger, Jr

Richard K Teague

Harriet Flannery Phillips

The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed inplants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

Production of a parvovirus vaccine in plants as viral coat protein fusions

A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Flexible processing apparatus for isolating and purifying viruses, soluble proteins and peptides from plant sources

A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.

Method for recovering proteins from the interstitial fluid of plant tissues

The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a larger scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target protein/peptide from other components of the green juice by one or more cycles of centrifugation and/or resuspension, and finally purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Process for isolating and purifying proteins and peptides from plant sources

The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

Parvovirus vaccine as viral coat protein fusions

The present invention features a method for isolating and purifying vitamins and sugars from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating vitamins and sugars than those methods described in the prior art. In general, the present method of isolating vitamins and sugars comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either vitamins or sugars, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying vitamins or sugars by such procedure as PEG-precipitation, chromatography and/or salt precipitation.

Process for isolating and purifying viruses and sugars from plant sources

Green note compound, such as cis-3-hexen-1-ol, is provided by subjecting linolenic acid and a fresh watermelon foliage to shearing in the presence of an aqueous liquid and yeast. Enzymes within the plant material (i.e., lipoxygenase and hydroperoxide lyase) and the yeast act to cause the linolenic acid to be converted to green note alcohol at a relatively high yield. Green note compound can be provided naturally using a continuous or batch process.

Method for providing green note compounds

The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspenion, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Process for isolating and purifying viruses, soluble proteins and

The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Growing community of inventors

Vacaville, CA, United States of America

Michael J McCulloch