Idec Pharmaceuticals Corporation

Biogen Idec, Inc.

Rit Oncology, LLC

Michael J LaBarre

Paul Chinn

Nabil Hanna

Kandasamy Hariharan

Gary R Braslawsky

John E Leonard

Steve Ruhl

Albert Gyorkos

Ronald A Morena

Thomas Ryskamp

Tri B Huynh

Antibody binding assays and radiolabeling kits are disclosed for radiolabeling and testing therapeutic antibodies in the commercial setting. In particular, the kits are designed for making and evaluating radiolabeled anti-CD20 conjugates to be used for the treatment and imaging of B cell lymphoma tumors. All kit reagents are sterile and are designed to achieve a high level of antibody radiolabeling and product stability with results which are highly reproducible.

Radiolabeling method

The present invention relates to a novel process for the preparation of biologically active antibody dimers in a pharmaceutically acceptable composition. The dimers can be composed of two antibody molecules having the same antigen binding specificity and linked through reducible, disulfide, or a non-reducible thioether, bond (homodimer). Alternatively, the dimers can be composed of two different antibody molecules having binding specificity for two distinct antigens (heterodimer). These dimers are useful for inducing hyper-cross-linking of membrane antigens. The present invention further relates to the use of biologically active antibody dimers for the preferential killing or inhibition of selected cell populations in the treatment of diseases such as cancer and autoimmune disorders.

Production of tetravalent antibodies

Disclosed are immunomagnetic electrochemiluminescence assays for eukaryotic cells, particularly human cells. A competitive binding assay is disclosed which enables affinity measurements of antibodies specific for eukaryotic cell membrane proteins. Such an assay is particularly useful for verifying or measuring the affinity of therapeutic antibodies following chelate conjugation.

Electrochemiluminescent assays for eukaryotic cells

The present invention provides a process for high yield regioselective synthesis of DTPA derivatives that eliminates the need for ion exchange chromotographic separation of intermediates. Moreover, as this process yields a single regioisomer of the chelate, it provides for the regiospecific synthesis of desired chelates useful as radiolabeling agents.

Regioselective synthesis of DTPA derivatives

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San Diego, CA, United States of America

Michael J LaBarre