Other

Emergence Genomics, LLC

Okura, Michael

Michael D Okura

Bruce J Richardson

Billy B McNeill

Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.

Systems, methods, and compositions for enhancing the specificity of nucleic acid hybridization

Methods and an apparatus according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are methods for improving accuracy and stringency of microarrays and/or other genomic analysis methods relying on nucleic acid hybridization and melting curve analysis by controlling surface chemistry. This method comprises producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, such as micro beads, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. Also provided is an improved microarray reader machine, to collect melting curve data on microarray slides. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.

Method for high accuracy genomic analysis utilizing hybridization and enhanced dissociation

A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector.

Method and apparatus for melting curve analysis of nucleic acids in microarray format

A tool for precisely determining the height and setback of a bicycle seat so that the seat can be adjusted to desired parameters. The tool can also be employed to determine the exact height of a bicycle's frame. The tool includes an adapter member that is attached to the bottom bracket spindle of the bicycle after removal of the crank arm. An elongate measuring pole is attached to the adapter member. Measuring indicia are inscribed on the outer surface of the pole. The measuring pole is provided with two components adjustably positioned along the length of the pole. One component is adapted to attach to the frame of the bicycle. The second component includes an adjustable position rod.

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Honolulu, HI, United States of America

Michael D Okura