H. Lundbeck A/s

Alderbio Holdings LLC

Akdeniz Universitesi

Mehmet Inan

John A Latham

Leon F Garcia-Martinez

Ethan Wayne Ojala

Patricia Dianne McNeill

Danielle Mitchell

Leon F Garcia-martinez

Fidan Erden Karaoglan

Mert Karaoglan

Danielle Marie Mitchell

The invention relates to the ADH3 promoter; polynucleotide sequences, vectors and expression cassettes including DNA regions responsible for the regulation of the ADH3 promoter; the host cells, including these vectors and expression cassettes, and, the recombinant proteins performed with the developed cells. In the scope of the invention, deletion analyzes in the ADH3 promoter were performed to identify regions that affect promoter strength and significant data was obtained in the formation of mutant ADH3 promoters. Deletion of the nucleotides between 539 and 638 (&#x2212;361 to &#x2212;262) in SEQ ID NO: 1 resulted in a 63% increase in ADH3 promoter activity. Five different synthetic promoters were created using positive regulatory regions identified and approximately 165% to 200% promoter activities were achieved with these promoters.

Determination of regulatory DNA regions of alcohol dehydrogenase 3 (ADH3) promoter and design of synthetic promoters for recombinant protein production

Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as

Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as

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Antalya, Turkey

Mehmet Inan