Wisconsin Alumni Research Foundation

Massachusetts Institute of Technology

Matthew D Shoulders

Ronald Thaddeus Raines

Jonathan Allen Hodges

Stephen J Lippard

Elizabeth Marie Nolan

Christopher Lawrence Moore

Samuel Joseph Hendel

Christopher E R Richardson

Chet Berman

Louis John Papa

Lisa S Cunden

Disclosed herein are methods of performing continuous directed evolution in complex biological systems, including metazoan cells. These methods include the infection of engineered, non-naturally occurring metazoan cells with engineered, non-naturally occurring DNA viruses. The generation of infectious viruses that can infect new cells depends on the evolution of a gene of interest which is driven by an error-prone adenoviral polymerase. Also disclosed herein, are the compositions of engineered, non-naturally occurring metazoan cells and engineered, non-naturally occurring DNA viruses that function as components in the continuous directed evolution methodologies.

Methods and compositions for performing continuous directed evolution

Novel collagen mimics are disclosed with a tripeptide unit having the formula (Xaa-Yaa-Gly), where one of the positions Xaa or Yaa is a bulky, non-electron withdrawing proline derivative. By substituting a proline derivative at either the Xaa or Yaa position in the native collagen helix, the stability of the helix is increased due solely to steric effects relative to prior known collagen-related triple helices. Methods are also disclosed for making the novel collagen mimics.

Collagen mimics

Compositions and methods for the selective sequestration of metal ions are generally described.

Compositions and methods for selectively sequestering metal ions

Novel collagen mimics are disclosed with a tripeptide unit having the formula (Xaa-Yaa-Gly),where one of the positions Xaa or Yaa is a bulky, non-electron withdrawing proline derivative. By substituting a proline derivative at either the Xaa or Yaa position in the native collagen helix, the stability of the helix is increased due solely to steric effects relative to prior known collagen-related triple helices. Methods are also disclosed for making the novel collagen mimics.

Growing community of inventors

Cambridge, MA, United States of America

Matthew D Shoulders