Other

Mathias Rainer

Douglas T Gjerde

Guenther Bonn

Christian Wolfgang Huck

Alexander Bonn

Bernhard Maerk

Fabio Polato

Harald Sonderegger

The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.

Isolation of phosphoproteins, glycoproteins and fragments thereof

The present invention relates to a pipette tip which is fitted with a porous organic monolith which is doped with active particles. Due to a unique polymerization method, the extraction tips stay highly permeable which allows sample to pass through the monolithic bed. The extraction tip represents an ideal tool for solid phase extraction, especially for desalting, isolating and purifying biomolecules such as peptides and proteins.

Pipette tip containing particle-filled polymer monolith

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Innsbruck, Austria

Mathias Rainer