Sanwa Kagaku Kenkyusho Co., Ltd.

Nippon Oil Fats Co., Ltd.

Kabushiki Kaisha Vitamin Kenkyuso

Shin-etsu Chemical Co., Ltd.

Sekisui Kagaku Kogyo Kabushiki Kaisha

Kabushiki Kaisha Vitamin Kenkyusyo

Institute of Applied Biochemistry

Kabushiki Kaisha Sanwa Kagaku Kenkyusho

Sawa Chemical Laboratory Co., Ltd.

Kabushiki Kaisha Vitamin Kenkyusho

Kabushiki Kaisha Vitamin Kenkyusya

Masayasu Kurono

Kiichi Sawai

Takahiko Mitani

Yasuaki Kondo

Ryoichi Unno

Toshinao Usui

Kenji Miura

Naohisa Ninomiya

Hiromoto Asai

Kyoichi Asano

Takuji Yamaguchi

Haruo Takahashi

Masato Fukushima

Hiromoto Kimura

Kunio Yagi

Makoto Sato

Kuniharu Mizuno

Akira Matsubara

Hiroshi Ozawa

Yoshiro Ishiwata

Yukiharu Matsumoto

Kenichi Tanaka

Noriaki Kato

Mitsuru Oka

Naofumi Terada

Tsunemasa Suzuki

Yutaka Baba

Tomoo Suzuki

Tomio Ogasawara

Takashi Ito

Nobuko Ohishi

Juichi Awaya

Noboru Kuboyama

Kazumasa Nakano

Takafumi Iida

Motohide Hayashi

Syoji Yokochi

Katsuya Fujimura

Kiyotaka Hirooka

Akio Kojima

Eiji Furukawa

Hisashi Michishita

Koichi Ito

Kunihiro Sumoto

Takahito Jomori

Seiji Miyano

Shigeshi Toyoshima

Takuji Kakigami

Shunji Natori

Yuji Hayashi

Takehiko Suzuki

Toshiyuki Kouzaki

Tsutomu Ishida

Hideaki Ninomiya

Takashi Nakagawa

Hitoshi Noda

Masahiro Nakajima

Takuya Koshizaka

Kazushi Tokita

Bunkichi Kato

Hiroyuki Ohwaki

Shizuo Uno

Shinichi Ikeda

Yohei Kobayashi

Jun Takeda

Hiroo Imura

Akira Tashita

Naohiro Kanayama

Noboru Tomiya

Masato Azuma

Kaneo Yamada

Hiroshi Okada

Yoshiya Kondo

Noriyuki Iwata

Katsuhiro Hayashi

Hiroko Tsukahara

Osamu Takehiro

Osamu Nakagawa

Sadaaki Iwamura

Tunematu Suzuki

Yoshinaru Tanaka

Hidehumi Yamakawa

Eiichi Kato

Tadashi Kurachi

Yutaka Seino

Bunichiro Yasui

Yukiya Tanaka

Naoko Sugiura

Shigeyoshi Nakamura

Mikio Minamitani

Takashi Meguro

Masashi Taki

Takao Matumoto

Katsuhiko Takahashi

Yuichi Shiokawa

Satoshi Uenoyama

Mitsuji Nakano

Keizo Anzai

Masanori Nagabuchi

Takashi Iida

For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1:2:2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.

Liposome for entrapping gene, liposomal preparation and process for the

There is disclosed a compound shown by a formula of ##STR1## wherein R.sub.1 is a hydrogen atom alkyl group having 1-4 carbon atoms or acyl group having 1-4 carbon atoms: R.sub.2 and R.sub.3 are a hydrogen atom, alkyl group having 1-4 carbon atoms, phenyl radical, halogen atom, cyano radical, acyl group having 1-4 carbon atoms, nitro radical, alkoxy group having 1 or 2 carbon atoms, or substituted or non-substituted amino group, respectively; n is an integer of 1-3; and dotted line means a possible ring, and a salt thereof. The compound and salt bind with muscarinic receptor in brain to develop a powerful actuation thereof and thus those can be used as an effective ingredient for preventing and curing senile dementias, and more particularly Alzheimer's disease.

Compounds and use thereof

A method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.

Method of enzymatically measuring hydrogen peroxide and reagent therefor

Process for the preparation of motilin-like polypeptide, a recombinant DNA therefor, and a plasmid, in which the recombinant DNA is inserted.

Process for the preparation of motilin-like polypeptide and expression

A readily absorbable type of motilin preparation containing a motilin-like active substance and a surface active agent is provided, in which the motilin-like active substance is mainly L-leucine-13-motilin-homoserine, and the surface active agent is selected from the group consisting of a bile salt, saponin and a polyethylene glycol higher-alcohol ether for nasal administration purposes and is a peptide lytic enzyme inhibitor for oral administration purposes.

Use of orally absorbable motilin preparations to enhance motility of

A process for the preparation of a polypeptide of the formula ##STR1## wherein A' is Pro, Gly, Asp or Ser; B' is Gly, Asn or Ser; C is Gln, Glu or Asp; D is Asn, Glu or Asp; X' is a residue of amino acid other than Met; E is Gln, Lys or Arg, and Y' is a residue of homoserine or homoserine-lactone, as well as a recombinant DNA and expression plasmid for preparing the polypeptide.

Motilin-like polypeptide and use thereof

Hydantoin derivatives and salts thereof, intermediates therefor, process for the preparation thereof, and medicines containing the derivative, wherein said derivatives have the formula ##STR1## wherein one of V and W is hydrogen and the other is a halogenomethyl group, 1H-tetrazol-5-yl radical, --COOR group,

Hydantoin derivatives for treating complications of diabetes

Early diagnosis of mastitis or garget is carried out by measuring the concentration of 3-hydroxybutyric acid in milk. Used to this end is a reagent reacting with 3-hydroxybutyric acid to show a color at a concentration of 100 .mu.mol/l or higher.

Early diagnosis of mastitis or garget

An aromatic amino-substituted compound represented by formula: ##STR1## wherein A represents CH, N, or N.fwdarw.O; R.sub.1 represents a nitro group or an amino group; R.sub.2 represents a hydrogen atom, a lower alkyl group, or an acyl group; and R.sub.3 represents a group: ##STR2## wherein m represents 0 or 1; n represents an integer of from 0 to 3; R.sub.4 and R.sub.5 each represents a hydrogen atom or a lower alkyl group; R.sub.6 and R.sub.7 each represents a hydrogen atom or a straight chain or branched lower alkyl group; R.sub.4 and R.sub.6 may be joined together to form an alkylene chain forming a heterocyclic ring; R.sub.5 and R.sub.7 may be joined together to form an alkylene chain forming a heterocyclic ring and R.sub.6 and R.sub.7 may be joined together to form an alkylene chain forming a heterocyclic ring,

Nootropic agents, compositions of, and method of use thereof

A pharmaceutical composition for stimulating peristaltic contraction in the intestinal canal which contains a pharmaceutically acceptable carrier or excipient and an effective amount of a polypeptide, or a salt thereof, having a pharmacological activity similar to motilin. The polypeptide has the formula B.sub.1 -Z.sub.1 -A.sub.1 -Z.sub.2 -B.sub.2 -Thr-B.sub.3 -A.sub.2 -Glu-Z.sub.3 -C-Arg-X'-Gln-Glu-Lys-Glu-Arg-D-Lys-Gly-E-Y, with Z.sub.1,Z.sub.2 and Z.sub.3 each being an amino acid residue having a hydrophobic side-chain, A.sub.1 and A.sub.2 each being an amino acid residue such as Pro, Gly, Asn and Ser, B.sub.1, B.sub.2 and B.sub.3 are each an amino acid residue having a side-chain with an aromatic ring, C is Gln, Glu or Asp, D is Asn, Glu or Asp, X' is an amino acid residue other than Met, E is Gln, Lys or Arg, and Y is homoserine, homoserine-lactone or a peptide chain having amino acid residues not exceeding 10 and containing homoserine or homoserine-lactone at the C-terminal where the first amino acid of said peptide chain is other than Lys, Arg or Pro.

Motlin-like polypeptide and use thereof

Vasoactive intestinal polypeptide (VIP) analogues and use thereof are disclosed. The VIP analogues are shown by the formula of

Vasoactive intestinal polypeptide analogues and use thereof

There is disclosed a pharmaceutical preparation which contains vasoactive intestinal polypeptide (VIP), its analogue or a salt thereof, and a surfactant. The preparation shall contains further a peptidase inhibitor. The composition is administrated to suppress allergies, reduce blood pressure, increase secretion of tear, cure asthma and impotence and others.

Pharmaceutical preparation containing vasoactive intestinal polypeptide

A process for separating and recovering serum and plasma components from a whole blood sample including passing a whole blood sample through a separating filter formed of a blood cell separating layer composed mainly of fibers impregnated with a coating agent. A device includes a blood collector having a needle at one end and a blood suction means at the other end, and a separating filter including a blood cell separating layer, the filter being provided in the intermediate zone of the blood collector, whereby a whole blood sample sucked through the needle by depressurizing the blood suction means is passed through the separating filter.

Process for separating serum and plasma

The invention provides a novel glycoside which contains as the aglycon a 4-(substituted phenyl)piperazine-1-yl derivative of glycyrrhetinic acid and 11-deoxo-glycyrrhetinic acid or their derivative as well as a composition for the treatment of virus infection, which contains these compounds as a main active component.

Glycyrrhetinic acid derivatives

A composition comprising an organogermanium compound represented by the formula ##STR1## wherein n is an integer of 1 or more, R is hydrogen, alkyl, --COOH, --COOR', phenyl, ##STR2## and R' is a lower alkyl and a high molecular carrier, as well as an immunity adjusting agent comprising the composition. The treatment of hepatitis with compositions containing this germanium derivative.

Composition containing organogermanium compound and immunity adjusting

Growing community of inventors

Mie, Japan

Masayasu Kurono