Nisshin Pharma Inc.

Kyoto University

Masataka Harata

Shingo Nonaka

Hiroyuki Ikemoto

Kenji Takemoto

Hideki Kanai

Sakayu Shimizu

Jun Ogawa

Shigeru Hiramoto

Eiji Sakuradani

Akinori Ando

Yuki Takemoto

Shirou Fujita

Yuto Inagawa

Ichiro Goto

Provided is a high-concentration and high-purity eicosapentaenoic acid-containing composition. A method for producing an eicosapentaenoic acid alkyl ester-containing composition, the method includes: (1) bringing a raw oil that contains an eicosapentaenoic acid alkyl ester into contact with an aqueous solution that contains a silver salt, and then collecting an aqueous layer; (2) adding an organic solvent to the aqueous layer, and then collecting an organic solvent layer; and (3) subjecting the organic solvent layer to vacuum distillation at a temperature of 180 to 188° C. and a tower top vacuum degree of 0.7 Pa or below, to recover the eicosapentaenoic acid alkyl ester from the organic solvent layer.

Eicosapentaenoic acid alkyl ester-containing composition and method for producing same

Provided is a method for producing a polyunsaturated fatty acid while suppressing deterioration of a silver salt solution. A method for producing a polyunsaturated fatty acid-containing composition, the method comprising: supplying a raw material solution comprising an alkyl ester of polyunsaturated fatty acid and an aqueous solution comprising a silver salt to a flow channel mixer to contact the raw material solution with the aqueous solution; and collecting the aqueous solution comprising the silver salt which has been contacted with the raw material solution, in which the supplying the aqueous solution comprising the silver salt to the flow channel mixer and the collecting the same are performed concurrently.

Method for producing polyunsaturated fatty acid-containing composition

Provided is a method for producing a polyunsaturated fatty acid while preventing deterioration of a silver salt solution. A method for producing a composition comprising a polyunsaturated fatty acid, comprising: supplying an aqueous solution comprising a silver salt into a reaction vessel to contact the aqueous solution with a raw material solution comprising an alkyl ester of the polyunsaturated fatty acid; and collecting the aqueous solution comprising the silver salt which has been contacted with the raw material solution from the reaction vessel, wherein the supply of the aqueous solution comprising the silver salt into the reaction vessel and the collection of the aqueous solution comprising the silver salt from the reaction vessel are carried out concurrently with each other.

Provided is3 desaturase having high enzymatic activity even at normal temperature. A polypeptide which consists of an amino acid sequence having an identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 2 and has3 desaturation activity on C20 fatty acid, and a gene thereof.

&#x3c9;3 fatty-acid desaturase and method for producing eicosapentaenoic acid

The present invention provides a composition having a high content of highly unsaturated fatty acid alkyl ester. A method for producing a composition comprising a highly unsaturated fatty acid alkyl ester, the method comprising contacting a raw material comprising a highly unsaturated fatty acid alkyl ester with an aqueous solution comprising a silver salt and subsequently recovering an aqueous phase; adding an organic solvent to the aqueous phase, and subsequently recovering an organic solvent phase; and rectifying the organic solvent phase at a temperature of 170 to 190&#xb0; C. and a column top vacuum degree of 1 Pa or less to recover the highly unsaturated fatty acid alkyl ester from the organic solvent phase.

Method for producing composition containing highly unsaturated fatty acid alkyl ester

Provision of a composition stably comprising a high concentration of sulfur amino acid derived from a plant belonging to the genus. A method for producing a sulfur amino acid-comprising composition comprising: heating a plant belonging to the genus; treating the plant belonging to the genusthus heated with a &#x3b3;-glutamyl bond cleaving enzyme; and subjecting the resulting enzyme-treated product to ion exchange chromatography.

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Ueda, Japan

Masataka Harata