Teijin Limited

Masami Fukuoka

Yataro Ichikawa

Tsukio Masegi

Kazuo Kitai

Satoshi Nakamura

Arata Kato

Jun Suzuki

Noriyuki Tsunekawa

Kenji Yone

Terminal modifications of tumor necrosis factor

A novel physiologically active polypeptide having antitumor activity obtained by improving human tumor necrosis factor (TNF). The amino acid sequence of the polypeptide essentially corresponds to that of human tumor necrosis factor, except that ten amino acids Nos. 1 to 10 are deleted and (Met).sub.n -Arg-Lys-Arg are added to the amino (NH.sub.2)-terminus, where n is 0 or 1. Other amino acid modifications can also be made to the polypeptide while retaining high antitumor activity. The novel polypeptide has less side effects that the natural-type TNF, particularly with respect to cytotoxicity. A a DNA fragment encoding the polypeptide, a recombinant plasmid containing the DNA fragment, a recombinant microorganism cell transformed with the plasmid, a method of producing a novel physiologically active polypeptide using the microorganism cell, and a pharmaceutical composition comprising the polypeptide as an active ingredient are also provided.

Muteins of tumor necrosis factor

Growing community of inventors

Hino, Japan

Masami Fukuoka