United States of America, As Represented by the Secretary of Agriculture

Mary M Maccree

William R Belknap

Kent F McCue

David R Rockhold

Paul V Allen

Louise V T Shepherd

Howard V Davies

Kenneth L Deahl

Teruko Osumi

Nucleic acid sequences from potato that encode the enzyme &#x3b2;-solanine/&#x3b2;-chaconine rhamnosyltransferase (SGT3) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT3 and thereby reduce levels of the predominant steroidal glycoalkaloids &#x3b1;-chaconine and &#x3b1;-solanine in Solanaceous plants such as potato are described.

[object Object]

Nucleic acid sequences from potato that encode the enzyme UDP-glucose:solanidine glucosyltransferase (SGT2) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT2 and thereby reduce the level of the more human-toxic of the two predominant steroidal glycoalkaloids &#x3b1;-chaconine and/or increase the level of the more insect-toxic &#x3b1;-solanine in Solanaceous plants such as potato are described.

Agene has been cloned from a late blight-resistance locus and its sequence provided, together with the sequence of the encoded protein. DNA encoding the resistance protein has been introduced into potato plants and confers resistance to, the causal agent of late blight. The resistance protein is in the class of Nucleotide Binding Site-Leucine-Rich Repeat Proteins (NBS-LRRP), and the gene inis flanked by related NBS-LRRP gene sequences.

Growing community of inventors

Woodland, CA, United States of America

Mary M Maccree