Monsanto Company

Mary M Hitt

Joseph Feder

William R Tolbert

Richard C Kimes

Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor.

Method of growing anchorage-dependent cells

A cell culture system and apparatus is provided for the agitated suspension culturing of mammalian cells in a flask assembly in which the culture medium and cells are gently agitated by the slow rotation of agitator means suspended downwardly in the culture medium and having at least one flexible sheet with a relatively large surface area which is positioned on the agitator in a manner to allow it to billow like a sail as liquid spills from its trailing edge during said rotation of the agitator means.

Cell culture method and apparatus

Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.

Growing community of inventors

St. Louis, MO, United States of America

Mary M Hitt