Pioneer Hi-bred International, Inc.

Mary J Frank

Shane E Abbitt

Bo Shen

Thomas W Greene

Jingrui Wu

Mary A Rupe

Shai J Lawit

Nandini Krishnamurthy

Rosana Melo

Andrew Rupiper

Genome edited plants, plant cells, seeds and plant parts of maize are provided where expression levels and/or activities of MADS-box transcription factors are modulated to improve one or more agronomic characteristics such as grain yield. Also provided are compositions comprising polynucleotides encoding polypeptides and guide RNAs targeted to endogenous maize MADS-box proteins including for example, targeted site-directed mutagenesis using CRISPR-associated nucleases. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a maize plant and methods for increasing yield of a maize plant are also provided herein.

Improving agronomic characteristics in maize by modification of endogenous mads box transcription factors

The disclosure relates to gene expression modulation elements from plants and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the expression modulating elements, and methods for preparing and using the same.

Expression modulating elements and use thereof

Growing community of inventors

Des Moines, IA, United States of America

Mary J Frank