Pioneer Hi-bred International, Inc.

Mary A Rupe

Mei Guo

Carl R Simmons

Bo Shen

Wesley B Bruce

Jingrui Wu

Rajeev Gupta

Jeffrey R Schussler

Jeffrey Habben

Olga Nikolaevna Danilevskaya

Nathan David Coles

Benjamin P Weers

Shoba Sivasankar

Shane E Abbitt

Xiaomu Niu

Shai J Lawit

Heather Marie Christensen

Mary J Frank

Rosana Melo

Olga Danilevskaya

Genome edited plants, plant cells, seeds and plant parts of maize are provided where expression levels and/or activities of MADS-box transcription factors are modulated to improve one or more agronomic characteristics such as grain yield. Also provided are compositions comprising polynucleotides encoding polypeptides and guide RNAs targeted to endogenous maize MADS-box proteins including for example, targeted site-directed mutagenesis using CRISPR-associated nucleases. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a maize plant and methods for increasing yield of a maize plant are also provided herein.

Improving agronomic characteristics in maize by modification of endogenous mads box transcription factors

The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Genes, constructs and maize event DP-202216-6

Methods for modulating plants using optimized ZmME293 down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear number and/or reduction in time to scenescence.

Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance

The present invention provides polynucleotides and related polypeptides of the ZmARGOS gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants.

Maize genes for controlling plant growth and organ size and their use in improving crop plants

The present invention provides polynucleotides and related polypeptides of the ZmERECTA gene family. The invention provides nucleic acid sequences for the Zm ERECTA genes. ZmERECTA is responsible for controlling plant growth, organ size and yield in crop plants.

Maize ERECTA genes for improving plant growth, transpiration, efficiency and drought tolerance in crop plants

The present invention provides polynucleotides and related polypeptides (SEQ ID NO: 11 and 44) of the ZmARGOS (Auxin-Regulated Gene involved in Organ Size) gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants. Transgenic plants expressing ZmARGOS show a positive impact on biomass accumulation and rate of maize plant growth, as well as an increase in organ size. These maize genes will find utility for enhancing agronomic traits in maize (and other crops).

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The present invention provides polynucleotides and related polypeptides (SEQ ID NO: 3 and 4) of the ZmARGOS (Auxin-Regulated Gene involved in Organ Size) gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants. Transgenic plants expressing ZmARGOS show a positive impact on biomass accumulation and rate of maize plant growth, as well as an increase in organ size. These maize genes will find utility for enhancing agronomic traits in maize (and other crops).

The present invention provides polynucleotides and related polypeptides (SEQ ID NO: 1 and 2) of the ZmARGOS (Auxin-Regulated Gene involved in Organ Size) gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants. Transgenic plants expressing ZmARGOS show a positive impact on biomass accumulation and rate of maize plant growth, as well as an increase in organ size. These maize genes will find utility for enhancing agronomic traits in maize (and other crops).

Zea maize auxin response related genes for controlling plant growth and organ size and their use in improving crop plants

The present invention provides polynucleotides and related polypeptides of the protein ZMNAM isolated from maize endosperm. The invention provides genomic sequence for the ZMNAM gene. ZMNAM is a novel gene and is regulated by gene-specific imprinting. ZMNAM expression is endosperm-specific and expressed throughout the endosperm development, peaking at 25 DAP. Genomic imprinting has been implicated to play a role in endosperm development. Genes regulated by allele-specific imprinting are suspected to be non-essential to seed development. Gene-specific imprinting however, regulates developmentally important genes. The ZMNAM gene, a putative transcriptional factor, may play an important role in endosperm development, which may further affect kernel size, plant vigor during germination and at the early seedling stage.

Growing community of inventors

Altoona, IA, United States of America

Mary A Rupe